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ÑÚ�D  4ýÇHv‹ß�¥4`pv	5O2Oœ¨O 'X]t	>�Í&º*‡ß	Ú+6NpÚ* ] &VWs›§¿=I¤_t	u	Ñ	Ò	Ó	Ô	Õ	Ö	×	Ø	Ù	Ú	Û	Ü	Ý	Þ	×ØÙ)*ÎÏÐ4MNŒ  +f  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
            	                                                                                                                          
                                                                                                                                                                                                                     
                       	                                                                                    	                     	       %               
                                                         	                  
                                    (                     o   ?               +      W   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
    	                                                
                                                                               
         	                              	       	   %     
                   	      
            (       o ?     +  W 
  aH  b.  bN  bŒ  eò  ið  oÂñ>]þ&'^>]o€"ÍÎßõ»þäÝ	í
›­Sji¨ *G¡0×"+&! é[˜
ÝzØšh.9~Dø
†ò–»… åAò¡ÊÓâé	…
±
Hºöz–ÈÏØöXÊ!´i}Mii¨ *;	 ŸÀú†¦‚‰>¾‰œDS¦æ		!z
í¾í	ü	
'à	Tí	õ	AÅÒÅ¿ÒGò®!	Êý#×wuý"n¯t…t…ý‹i¦žpäã æ 02‰õl‹
ÔÎ	U
tÿFOé^È ÿ ¸äN	ê
Œg�B�§­š­²)‰õ  žÎ’Ù=‹Ÿ Ø è €ÅN£”è Å£”À€}!ä û	ÿ	¥
à
³Šd è /ÜmÅ£”È äÃ‚ ó €Ó8 ‰õÎX\S‹ß5OÔ(aá	
”Sù�‰õßÎy ô 0T„2G‰õÃÏñýþ‚‰lƒ‹
©ÐÔ2[.|ÎÝ=X]	
ûƒ3	õtw÷K�)] ª LdˆÁý]�DÈ‹úýÁa©†
+E÷Ÿ pYì+>èôŸ U(î	Xç	» ©ÅBmz	 cÓ»é¡ 	4Üw÷àª ]Èa è ó /€ÜmÅN£”h[K´‹ª Lˆ]Èaƒ|# d r (È·»_ã	ç	î	º
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Dš¿ïvkÿª ]Èaµ‹yÃ$a‚­KmyyŠ‘–¶
­ö	€“GÅÓe¡ €B�§­Ý=Xš­]²#ô—�ùš†«è ú SðÅ­£”
Ìp|™ fÁíãñ		4	Ç
tú\¾[^K´ÒTAI(î	t ìÑÙ
ßÔ�SCPƒ}Ô(aá	ìÑÙ
}ƒ3		
õût*2‹2¦3nÕÑhˆæŠU	ü 	¡		Ãaån‚ð­KmyÄ%ƒÂzJ‹dþÀŒúý!u†�Á©†
¦,E–hG	Há÷.Kv +ëîÐÓÝÛ
,>á"%ó;©á*q  TGHFËòþ$ôª2�’pØ^ òï¾5® Gò%iâÓïø°°
sVÄòþôª’pëÐÝ"%ó©v +îÓÛ
,>á;á*Ë ,5,¨ÇÃX)þ ‚èE‡´qfo v "ë+0·ëîJYÐÓÝ		ë	Û
,>A·Rá"%ó;Ž©íyá*^ dþòÀŒïúý!u†�Á©†
¦,¾5Eãÿ·|ÈjÏÑ1³x‹+ßàÃ
 6¬×ŽPjx(0$k¤rô©È; vvBÀ6Þ!ž7ÝÆ;õÓùŽgñqÿâr	ö6/e¬‚»¾öé; } � vÆvB;Àõ6ÓÞ!K	ž7Ýù£Ž9Î7’k–¶
#*­Â ñ3!Os œ»
ô
U_ÿtšùHv›ÀE	æ
'rþ{CÁð
Wd< Aqw:¯7R8BÈ"˜ûIª0·u€ø^»l“q§k� D‘¯â~âñHóù¯™"	
ð/£¾	CÜ˜?S“ – UXƒÀ"U|ÉÌƒ}º£	pc±E	þtù›'{Hóù¯™
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<Created UTC="16:0:0">1999.7.15</Created>
<LastModified UTC="6:21:5">2021.4.23</LastModified>
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<CreatedBy>Promega</CreatedBy>
<Organism>Escherichia coli</Organism>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGEMHE-NLS-mCherry-mTrim21</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector for standard cloning, efficient &lt;i>in vitro&lt;/i> RNA synthesis, and ssDNA production.&amp;nbsp;Identical to&amp;nbsp;pGEMÂ®â€‘3Zf(-) except for the orientation of the f1 origin.&lt;/body>&lt;/html></Description>
<Created UTC="16:0:0">1999.7.15</Created>
<LastModified UTC="19:33:2">2025.9.3</LastModified>
<AccessionNumber>X65306</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
<Organism>Escherichia coli</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>&lt;html>&lt;body>&lt;br>&lt;/body>&lt;/html></Comments>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  K              E              ÿÿÿÿYô? P                                                                                                                                                             <StrandColors><TopStrand><ColorRange range="0..105" colors="black"/><ColorRange range="159..5253" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..105" colors="black"/><ColorRange range="159..5253" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
